The 35 works included Rothko’s 8ft tall Number 7, which was sold for $82.5m, the largest single sum of the sale.
The couple were renowned for making barbed exchanges at public events, and their marital strife eventually resulted in a divorce.
The sale at Sotheby’s in New York on Monday evening was regarded as the biggest test of the fine art market since the start of the Covid pandemic in early 2020.